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Image Search Results
Journal: The Journal of Biological Chemistry
Article Title: The structure of the archaeal nuclease RecJ2 implicates its catalytic mechanism and inability to interact with GINS
doi: 10.1016/j.jbc.2024.107379
Figure Lengend Snippet: The determination of protein molecular weight using size exclusion chromatography coupled with multiangle light scattering (SEC-MALS). The molar masses calculated from the SEC-MALS analysis are represented by the red line. The graph also shows the Refractive Index (RI) signal, which correlates with the concentration of the protein in the eluate. By analyzing the intensity of scattered light at multiple angles, SEC-MALS provides an accurate measurement of the molecular weight, independent of the protein's shape and size in solution.
Article Snippet: The molecular weight measurements for MjRecJ2-WT and MjRecJ2-M dissolved in the solution were conducted using a
Techniques: Molecular Weight, Size-exclusion Chromatography, Multi-Angle Light Scattering, Refractive Index, Concentration Assay